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人工合成的小口径血管支架亟需攻克抗凝血和快速内皮化两大难题。负载有机硒催化剂聚乙烯亚胺(SePEI)能够催化一氧化氮(NO)的原位生成,从而有效地抑制血栓形成。层粘连蛋白来源多肽Tyr-Ile-Gly-Ser-Arg(YIGSR)具有内皮细胞选择性粘附功能,负载到支架表面能够诱导内皮层的快速形成。本实验采用静电纺丝构建聚己内酯(PCL)支架,通过层层自组装构建Se PEI和透明质酸(HA)的双电层催化NO的生成;最外层的HA接枝有YIGSR多肽促进内皮化。考察和比较了材料修饰前后的物化性能。修饰过程对纤维形貌无明显影响,负载稳定性经过65d仍能保持在99%以上。材料经修饰后力学性能有所提高,能够满足作为血管支架的要求,为下一步细胞培养和体内实验打下基础。
Synthetic small caliber vascular stents urgently need to overcome the two major problems of anticoagulant and rapid endothelialization. SePEI loaded with organic selenium catalyst can catalyze the in situ generation of nitric oxide (NO), thus effectively inhibiting thrombosis. The laminin-derived polypeptide Tyr-Ile-Gly-Ser-Arg (YIGSR) has endothelial cell selective adhesion function, which can be loaded onto the scaffold surface to induce the rapid formation of endothelial cells. In this experiment, poly (L-caprolactone) (PCL) scaffolds were constructed by electrospinning. The double layer of Se PEI and hyaluronic acid (HA) was used to catalyze the formation of NO by layer self-assembly. The outermost layer of HA was grafted with YIGSR polypeptide Promote endothelialization. The physical and chemical properties before and after modification were investigated and compared. Modification of the fiber morphology had no significant effect, load stability after 65d can still be maintained at more than 99%. The mechanical properties of modified materials have been improved to meet the requirements of vascular stents, laying the foundation for the next step of cell culture and in vivo experiments.