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目的 观察氧化电位水 (EOW)在胃镜消毒中对乙型肝炎表面抗原 (HBsAg)抗原性的破坏效果。方法 模拟现场试验用EOW与人工污染的胃液作用 1min ,观察其对HBsAg的破坏作用 ,以PBS作为对照组。现场试验以HBsAg阳性病人用后的污染胃镜 ,作为消毒前对照 ,经去有机物处理及EOW浸泡 1min ,胃镜表面涂抹采样 ,用ELISA检测HBsAg。结果 人工污染胃液 (HBsAg平均S/N值为 42 .16 )加入胃液量 10 0倍的EOW作用 1min后 ,HBsAg检测为阴性 ,对照组仍为阳性。临床污染胃镜 (HBsAg平均S/N值为5 .99)经清洁、消毒后HBsAg检测为阴性。 结论 在清除有机物后 ,氧化电位水可有效的破坏污染胃镜HBsAg的抗原性
Objective To observe the destroying effect of oxidation potential water (EOW) on the antigenicity of hepatitis B surface antigen (HBsAg) in gastroscopic disinfection. Methods The field experiments were conducted with EOW and artificial contaminated gastric juice for 1 min to observe the destructive effect of HBsAg on HBsAg. PBS was used as the control group. Field experiments with HBsAg-positive patients with contaminated gastroscopy, as a pre-disinfection control, go to organic treatment and EOW soak 1min, gastroscope surface smear sampling, ELISA detection of HBsAg. Results The results of artificial contamination of gastric juice (HBsAg average S / N value of 42.16) added to the amount of gastric juice EOW 1min after 1min, HBsAg test was negative, the control group was still positive. Clinical contamination of gastroscope (average HBsAg S / N value of 5.99) after cleaning, disinfection HBsAg negative. Conclusion After the removal of organic matter, oxidation potential water can effectively destroy the antigenicity of gastroscope HBsAg