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目的观察生存素(survivin)蛋白在不同胃组织中的表达与肿瘤细胞凋亡及增殖的关系。方法采用免疫组织化学SP法检测生存素蛋白,DNA末端标记法TUNEL技术检测细胞凋亡。结果150例胃癌标本中有73例生存素蛋白阳性表达,其阳性率48.67%;生存素蛋白阳性表达组中的凋亡指数(0.60%)与其阴性组(0.94%)之间有统计学差异(P<0.05);增殖指数也明显高于阴性组(P<0.05);生存素蛋白在正常胃黏膜中没有表达,在上皮内瘤变中生存素蛋白有不同程度的表达,与正常胃黏膜之间有统计学意义(P<0.05),低级别与高级别上皮内瘤变之间有统计学意义(P<0.05),高级别上皮内瘤变与胃癌之间无统计学意义(P>0.05)。从低级别上皮内瘤变、高级别上皮内瘤变到胃癌的过程中,凋亡指数逐渐减少,增殖指数逐渐增加。结论生存素蛋白抑制胃癌细胞凋亡,促进胃癌细胞增殖,与肿瘤的发生有关。
Objective To investigate the relationship between the expression of survivin protein in different gastric tissues and the apoptosis and proliferation of tumor cells. Methods Survivin was detected by immunohistochemical SP method and apoptosis was detected by TUNEL technique. Results The positive rate of survivin in 73 cases of gastric cancer specimens was 48.67%. The apoptotic index in survivin positive group (0.60%) was significantly lower than that in negative group (0.94%) ( P <0.05). The proliferation index was also significantly higher than that in the negative group (P <0.05). Survivin protein was not expressed in normal gastric mucosa and survivin protein was expressed in varying degrees in intraepithelial neoplasia, (P <0.05). There was a significant difference between low-grade and high-grade intraepithelial neoplasia (P <0.05). There was no significant difference between high-grade intraepithelial neoplasia and gastric cancer (P> 0.05) ). From the low-grade intraepithelial neoplasia, high-grade intraepithelial neoplasia to gastric cancer process, the apoptotic index gradually decreased, the proliferation index increased gradually. Conclusion Survivin can inhibit the apoptosis of gastric cancer cells and promote the proliferation of gastric cancer cells, which is related to the tumorigenesis.