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酸性转化酶参与了番木瓜果实中蔗糖的韧皮部卸载,调控果实中糖类的积累。本文利用番木瓜基因组数据库信息,发现了番木瓜基因组中含有6个酸性转化酶基因,分别为细胞壁转化酶基因(CpCWINV1-3)和液泡转化酶基因(CpVINV1-3)。这些基因由3~7个外显子组成,其中CpCWINV1、CpVINV1和CpVINV2的N端均具有一个跨膜区域。蛋白质3D结构预测分析表明,CpCWINV1、CpVINV1和CpVINV2具有典型的N端β-螺旋桨模块(β-propeller module)和C端β-三明治模块(β-sandwich module),而CpCWINV3和CpVINV3的桨叶I上,均没有保守区NDPNG/A;CpCWINV2和CpVINV3均缺失了β-三明治模块。推测番木瓜细胞壁转化酶CpCWINV1、液泡转化酶CpVINV1和CpVINV2能正确履行催化蔗糖分解的功能。本研究有助于我们进一步揭示酸性转化酶在番木瓜果实发育及糖类积累过程中的作用。
Acid invertase participates in the unloading of phloem in papaya fruits and regulates the accumulation of sugars in fruits. In this paper, papaya genome database information found in the papaya genome contains six acid invertase gene, respectively, the cell wall invertase gene (CpCWINV1-3) and the vacuolar invertase gene (CpVINV1-3). These genes consist of 3 to 7 exons, of which CpCWINV1, CpVINV1 and CpVINV2 each have a transmembrane region at the N-terminus. Prediction of protein 3D structure showed that CpCWINV1, CpVINV1 and CpVINV2 have typical β-propeller module and β-sandwich module, while CpCWINV3 and CpVINV3 have a , There is no conserved region NDPNG / A; CpCWINV2 and CpVINV3 are missing β-sandwich module. It is speculated that papaya cell wall invertase CpCWINV1, vacuolar invertases CpVINV1 and CpVINV2 can correctly perform the function of catalyzing sucrose decomposition. This study will help us further reveal the role of acid invertase in the development of papaya fruit and carbohydrate accumulation.