目的　检测人子宫内膜癌中抑癌基因p5 3基因突变的情况并探讨其与临床病理特征的关系。方法　采用聚合酶链反应 (PCR)和限制性酶切片段长度多态性分析 (RFLP)技术检测 40例子宫内膜癌中p5 3基因第 6、第 7外显子的突变。结果　检出突变 7例 ,突变与病理学分级有关 ,与临床分期、绝经情况、组织学类型无关。结论　p5 3基因突变可能是子宫内膜癌发生的原因之一 Objective To detect the mutation of tumor suppressor gene p5 3 in human endometrial carcinoma and to explore its relationship with clinicopathological features. Methods The mutations of exon 6 and exon 7 of p5 3 gene in 40 cases of endometrial carcinoma were detected by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). Results 7 cases were detected mutations, the mutation was related to the pathological grade, and clinical stage, menopause, histological type has nothing to do. Conclusion The mutation of p5 3 gene may be one of the causes of endometrial carcinoma