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采用花粉管通道法将ATNCED3基因转入优良玉米自交系昌7-2,在T0代植株三叶期喷洒浓度为200mg/L草铵膦除草剂进行抗性筛选,共得到5株草铵膦抗性植株。对这5株草铵膦抗性植株进行PCR、PCR-Southern及RT-PCR检测,结果表明5株均为阳性植株,初步证明外源目的基因已整合到玉米基因组中,并得到表达。在干旱条件下初步比较了对照和转基因株系叶片的相对含水量、脯氨酸含量,相对电导率等生理生化指标,结果表明,转基因株系各种指标均好于对照,暗示其抗旱性有所提高。
The ATNCED3 gene was transferred into Chang 7-2, an elite maize inbred line, by pollen tube pathway. The three-leaf stage T0 plants were sprayed with a concentration of 200 mg / L of glufosinate-ammonium herbicide to screen for 5 strains of glufosinate Resistant plants. The five glufosinate-resistant plants were tested by PCR, PCR-Southern and RT-PCR. The results showed that all the five plants were positive plants. The results showed that the exogenous gene was integrated into the genome of maize and expressed. The relative water content, proline content, relative conductivity and other physiological and biochemical indexes of the leaves of the control and transgenic lines were compared under drought conditions. The results showed that all the indexes of the transgenic lines were better than the control, indicating that the drought resistance Increased.