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鞭毛染色法,实为细菌学技术操作较为困难的一项工作,染色方法颇多,有些较难得到满意之结果,而对初学者更不易于掌握,往往易遭失败,据本室研究之新染色法,与其他数种染色法之比较,认为本法较佳,笔者在染色方法中所体会的一点经验和要点,介绍如下,以供初学者之参考,我们染了伤寒桿菌“H”,副伤寒桿菌“甲”,副伤寒桿菌“乙”,大肠桿菌,变形桿菌,粪产龄桿菌及绿脓桿菌等,上述各种细菌按照下列手术进行操作,即可得到满意之结果。一、脱脂玻璃片的清洁法取无纹之新玻片,用肥皂水煮沸30分钟,以热水冲洗数次,彻底洗净,用软布擦干,以去油质,
Flagella staining is a difficult task for bacteriological technology. There are many staining methods. Some of them are more difficult to obtain satisfactory results. They are less easy to master for beginners and are often susceptible to failure. According to the new research in this room, The staining method, compared with several other staining methods, is considered to be better. The experience and points learned by the author in the dyeing method are described below. For reference by beginners, we infected the typhoid bacillus “H”. Paratyphoid bacillus “A”, paratyphoid bacillus “B”, E. coli, Proteus, Bacillus faecalis, and Pseudomonas aeruginosa, etc. The above-mentioned various bacteria can be operated according to the following operations, and satisfactory results can be obtained. First, clean the skim glass sheet Take a new glass slide without a grain, boil it in soapy water for 30 minutes, rinse it several times with hot water, wash it thoroughly, dry it with a soft cloth to remove oil,