目的 :研究重组人粒细胞巨噬细胞集落刺激因子(recombinant human granulocyte/macrophage colonystimulating factor,rhGM-CSF)与重组人粒细胞集落刺激因子(recombinant human granulocyte colony-stimulating factor,rhG-CSF)对肺腺癌A549细胞顺铂化疗敏感性的影响。方法 :采用免疫细胞化学法检测A549细胞中GM-CSF及G-CSF受体的表达情况。CCK-8法检测不同浓度rhGM-CSF、rhG-CSF和顺铂对A549细胞增殖的影响,以选择最适浓度进行A549细胞对顺铂联合rhGM-CSF或rhG-CSF化疗敏感性的研究;分别采用CCK-8法、FCM法和Transwell小室法检测rhGM-CSF和rhG-CSF对A549细胞顺铂化疗敏感性的影响。结果 :GMCSF和G-CSF受体在A549细胞上均有表达;rhGM-CSF和rhG-CSF在质量浓度为0.1 ng/mL时促进A549细胞增殖的作用最明显;顺铂在质量浓度2.0μg/mL时对A549细胞的的抑制率为(34.46±2.07)%,4.0μg/mL时抑制率为(65.91±2.78)%,因此选择顺铂浓度为2.0μg/mL,rhGM-CSF和rhG-CSF浓度为0.1 ng/mL进行序贯治疗。顺铂序贯rhGM-CSF组中,与顺铂单药组相比,顺铂联合rhGM-CSF能提高顺铂对A549细胞增殖的抑制作用,但差异无统计学意义;同时能明显增强顺铂诱导A549细胞凋亡,抑制细胞迁移和侵袭的能力,差异均有统计学意义(P<0.05)。而顺铂序贯rhG-CSF组中,与顺铂单药组相比,顺铂联合rhG-CSF明显降低了顺铂抑制A549细胞增殖、诱导细胞凋亡、抑制细胞迁移和侵袭的作用,差异均有统计学意义(P<0.05)。结论 :rhGM-CSF或可增加A549细胞对顺铂的敏感性,而rhG-CSF则可减弱其对顺铂的敏感性。 Objective: To investigate the effects of recombinant human granulocyte / macrophage colony stimulating factor (rhGM-CSF) and recombinant human granulocyte colony-stimulating factor (rhG-CSF) Effect of chemosensitivity of cisplatin on A549 cells. Methods: Immunocytochemistry was used to detect the expression of GM-CSF and G-CSF receptor in A549 cells. The effects of rhGM-CSF, rhG-CSF and cisplatin on the proliferation of A549 cells were detected by CCK-8 assay. The sensitivity of A549 cells to cisplatin combined with rhGM-CSF or rhG-CSF chemotherapy was determined by selecting the optimal concentration. The effects of rhGM-CSF and rhG-CSF on cisplatin chemosensitivity of A549 cells were detected by CCK-8, FCM and Transwell chamber methods. Results: GMCSF and G-CSF receptor were all expressed on A549 cells. The effect of rhGM-CSF and rhG-CSF on the proliferation of A549 cells was most obvious when the concentration of rhGM-CSF and rhG-CSF was 0.1 ng / mL. mL, the inhibitory rate of A549 cells was (34.46 ± 2.07)% and that of 4.0μg / mL was (65.91 ± 2.78)%. Therefore, the concentration of cisplatin was 2.0μg / mL and rhGM-CSF and rhG-CSF The concentration was 0.1 ng / mL for sequential therapy. Compared with the cisplatin monotherapy group, cisplatin combined with rhGM-CSF can increase the inhibitory effect of cisplatin on the proliferation of A549 cells, but the difference was not statistically significant; at the same time, the cisplatin The apoptosis of A549 cells was significantly inhibited (P <0.05). The difference was statistically significant (P <0.05). The cisplatin rhG-CSF group, compared with the cisplatin monotherapy group, cisplatin combined with rhG-CSF significantly reduced the proliferation of A549 cells induced by cisplatin, induced apoptosis, inhibition of cell migration and invasion, the difference All were statistically significant (P <0.05). Conclusion: rhGM-CSF can increase the sensitivity of A549 cells to cisplatin, while rhG-CSF can reduce its sensitivity to cisplatin.