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以辣椒核型雄性不育两用系 AB154和 AB 兖1691为试材,对不育株和可育株小孢子的不同发育时期进行了细胞学比较分析。结果表明:不育株小孢子败育发生在四分体小孢子形成以前,靠近小孢子的一层绒毡层细胞在小孢子母细胞期已解体,其余绒毡层细胞在花粉粒成熟期仍然结构完整;可育株绒毡层在单核花粉粒期开始解体,花粉粒成熟期时完全解体。不育株花药药隔维管束发育滞后于可育株,退化却早于可育株。部份绒毡层提前解体和药隔维管束发育异常使小孢子正常发育所需要的营养物质供应受阻,可能是造成小孢子败育的重要原因。
Cytological analysis of different developmental stages of sterile and sterile microspores was carried out by using AB154 and AB Yanhua strains with pepper karyotype as male sterile lines. The results showed that the sterile microspore abortion occurred before the formation of tetrad microspores. The tapetum cells near the microspores had disintegrated during the microspore mother cell stage, while the remaining tapetum cells remained at the maturity stage of pollen grains The structure was intact. The tapetum of fertile plants began to disintegrate at the pollen stage of mononuclear pollen and the pollen grains disintegrated completely at maturity. Sterile plants anther drug vascular bundle lagging behind the fertile plants, degradation was earlier than the fertile plants. Part of the tapetum in advance disintegration and drug vascular bundle abnormalities so that the normal development of microspore nutrients needed to block the supply may be an important reason for the formation of microspore abortion.