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作者报道反转录聚合酶链式反应(RT-PCR),双单克隆抗体(McAb)夹心法酶联免疫吸附试验(SandwichELISA)和间接免疫荧光技术(IFA)三种方法对汉坦病毒76~118株细胞培养物中病毒RNA及抗原的检测情况,并比较了三种方法的敏感性.结果表明:RT-PCR敏感性最高,种毒后24h内即可检出病毒RNA;其次为夹心法ELISA,种毒后48h可检出冻融细胞上清内的病毒抗原;IFA检出感染细胞内病毒抗原的最短时间为72h.作者对设计引物与提高RT-PCR的敏感性进行了讨论。
The authors reported three methods of reverse transcription-polymerase chain reaction (RT-PCR), sandwich ELISA and Sanda ELISA (indirect immunofluorescence assay) 118 cell cultures of viral RNA and antigen detection, and compared the sensitivity of the three methods. The results showed that RT-PCR had the highest sensitivity and could detect the virus RNA within 24 hours after being infected with the virus. The second was sandwich ELISA, and the virus antigen in the supernatant of frozen-thaw cells could be detected 48 h after being infected. IFA detected the infected cells The shortest time for virus antigen is 72h. The authors discussed the design of primers and their sensitivity to improve RT-PCR.