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目的探讨卡波西肉瘤细胞系转染微小RNA-126(microRNA-126,miR-126)后对细胞迁移和侵袭能力影响。方法卡波西肉瘤细胞株SLK随机分为miR-126模拟物组、阴性对照组、miR-126抑制物组和抑制物阴性对照组,分别将miR-126模拟物25ng、阴性对照25ng、miR-126抑制物250ng和抑制物阴性对照250ng加入转染试剂3μL,室温孵育10min形成转染混合物,分别转染到4组细胞悬液中,孵育48h后行Transwell小室迁移实验和侵袭实验,将Transwell小室基底膜完整切除,行苏木精染色,光学显微镜下计数细胞迁移数目和细胞侵袭数目。结果 miR-126模拟物组细胞迁移数目[(77.33±7.02)个]和细胞侵袭数目[(25.67±2.52)个]较阴性对照组[(96.67±6.11)、(45.67±2.08)个]、miR-126抑制物组[(138.00±8.00)、(76.67±4.16)个]和抑制物阴性对照组[(101.33±9.29)、(47.33±1.53)个]少(P<0.05);miR-126抑制物组细胞迁移和侵袭数目较阴性对照组和抑制物阴性对照组多(P<0.05);阴性对照组细胞迁移和侵袭数目与抑制物阴性对照组比较差异无统计学意义(P>0.05)。结论 miR-126对卡波西肉瘤细胞侵袭和迁移起抑制作用,可能成为卡波西肉瘤的潜在治疗靶点。
Objective To investigate the effect of microRNA-126 (miR-126) on the migration and invasion of Kaposi’s sarcoma cell line. Methods Kaposi’s sarcoma cell line SLK was randomly divided into miR-126 mimics group, negative control group, miR-126 inhibitor group and inhibitor negative control group. The miR-126 mimics 25 ng, 25 ng negative control and miR- 126 inhibitor 250ng and inhibitor negative control 250ng added transfection reagent 3μL, incubated at room temperature for 10min to form a transfection mixture were transfected into four groups of cell suspension 48h incubation after Transwell chamber migration experiments and invasion experiments Transwell chamber Basement membrane complete resection, hematoxylin staining, counting the number of cell migration and cell invasion under light microscope. Results Compared with the negative control group [(96.67 ± 6.11), (45.67 ± 2.08)], the number of miR-126 mimics group [(77.33 ± 7.02) (P <0.05); miR-126 inhibitor group [(138.00 ± 8.00) vs (76.67 ± 4.16)] and inhibitor negative control group (101.33 ± 9.29), (47.33 ± 1.53) The numbers of cell migration and invasion were higher in the control group than those in the negative control group and the negative control group (P <0.05). There was no significant difference in the numbers of cell migration and invasion between the negative control group and the negative control group (P> 0.05). Conclusion miR-126 inhibits the invasion and migration of Kaposi’s sarcoma cells and may be a potential therapeutic target for Kaposi’s sarcoma.