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目的:建立HPLC法测定蒙药漏芦花中槲皮素-3-O-α-L-鼠李糖苷、木犀草素、芹菜素、5,7,4′-三羟基-3′-甲氧基黄酮、蜕皮甾酮和泥胡木脂素B葡萄糖苷的含量。方法:采用YMC-Pack C_(18)色谱柱(4.6 mm×250 mm,5.0μm),以乙腈(A)-0.2%磷酸水溶液(B)为流动相,梯度洗脱,流速0.8 m L·min~(-1),柱温35℃,检测波长254 nm。结果:槲皮素-3-O-α-L-鼠李糖苷、木犀草素、芹菜素、5,7,4′-三羟基-3′-甲氧基黄酮、蜕皮甾酮和泥胡木脂素B葡萄糖苷6个成分的进样量分别在0.090~2.24μg(r=1.000 0)、0.048~1.19μg(r=0.999 8)、0.040~1.00μg(r=0.999 9)、0.008~0.20μg(r=0.999 8)、0.080~2.00μg(r=1.000 0)、0.092~2.31μg(r=1.000 0)范围内线性关系良好;平均回收率在99.2%~103.9%范围内,RSD范围为0.82%~2.9%。8批漏芦花样品中槲皮素-3-O-α-L-鼠李糖苷、木犀草素、芹菜素、5,7,4′-三羟基-3′-甲氧基黄酮、蜕皮甾酮和泥胡木脂素B葡萄糖苷的含量范围分别为0.66%~1.26%、0.19%~0.60%、0.08%~0.24%、0.03%~0.05%、0.31%~0.99%、0.42%~2.26%。结论:该方法可以用于蒙药漏芦花中6个指标性成分的含量测定,为漏芦花药材的质量控制提供科学依据。
Objective: To establish a HPLC method for the determination of quercetin -3-O-α-L-rhamnoside, luteolin, apigenin, 5,7,4’-trihydroxy-3’-methoxyflavone , Ecdysterone and mud lignans B glucoside content. Methods: The mobile phase consisted of a gradient of YMC-Pack C_ (18) (4.6 mm × 250 mm, 5.0 μm) with acetonitrile-A0.2% phosphoric acid solution ~ (-1), column temperature 35 ℃, detection wavelength 254 nm. Results: Quercetin -3-O-α-L-rhamnoside, luteolin, apigenin, 5,7,4’-trihydroxy-3’-methoxyflavone, ecdysterone, The injection volumes of 6 components of adiponectin B glucoside were 0.090-2.24μg (r = 1.000 0), 0.048-1.19μg (r = 0.999 8), 0.040-1.00μg (r = 0.999 9), 0.008-0.20 (r = 0.999 8), 0.080 ~ 2.00μg (r = 1.000 0), 0.092 ~ 2.31μg (r = 1.000 0). The average recoveries ranged from 99.2% to 103.9% 0.82% ~ 2.9%. 8 batch of aloe samples of quercetin -3-O-α-L-rhamnoside, luteolin, apigenin, 5,7,4’-trihydroxy-3’- methoxy flavone, ecdysterone And the content of mud lignin B glucoside ranged from 0.66% to 1.26%, from 0.19% to 0.60%, from 0.08% to 0.24%, from 0.03% to 0.05%, from 0.31% to 0.99% and from 0.42% to 2.26%, respectively. Conclusion: This method can be used for the determination of 6 index components in Mongolian medicine, and provide a scientific basis for the quality control of