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目的观察蒙古沙鼠感染幽门螺杆菌(Helicobacter pylori,H.pylori)后胃部菌群及病理学变化。方法 5周龄蒙古沙鼠60只,随机分为实验组(30只)和对照组(30只)。所有沙鼠禁食不禁水24 h后,实验组灌喂109CFU/mLH.pylori菌液0.5 mL/只,连续3次。对照组灌喂无菌肉汤。在4、8、16、24和48周处死动物,进行胃部菌群分析和H.pylori分离培养及病理学检查。结果正常沙鼠胃中存在着以乳酸菌为主的正常菌群[(8.43±5.21)×105CFU/g],感染H.pylori后正常菌群数量显著减少;实验组沙鼠H.pylori感染率为100%,第4周可见沙鼠胃组织红肿充血,第8周有炎性细胞浸润,16周和24周出现糜烂,48周见出血、慢性活动性胃炎及溃疡。对照组沙鼠无H.pylori定植及组织学病变。结论 H.pylori感染使蒙古沙鼠胃内正常菌群发生变化,从而引起胃炎和胃溃疡发生。
Objective To observe the changes of gastric flora and pathology in gerbils infected with Helicobacter pylori (H.pylori). Methods Sixty Mongolian gerbils, aged 5 weeks, were randomly divided into experimental group (n = 30) and control group (n = 30). All fasting mice could not help but water 24 h, the experimental group fed 109CFU / mLH.pylori bacteria 0.5 mL / only, 3 times in a row. The control group was fed sterile broth. The animals were sacrificed at weeks 4, 8, 16, 24 and 48 for analysis of stomach flora and H.pylori isolation and pathology. Results The normal bacterial flora mainly existed in the stomach of normal gerbils [(8.43 ± 5.21) × 105CFU / g], and the number of normal flora decreased significantly after H. pylori infection. The infection rate of H.pylori in gerbils in experimental group was 100%. In the 4th week, gerbils’ gastric tissues were inflamed and inflamed, the infiltration of inflammatory cells was observed in the 8th week, erosion occurred at 16th week and 24th week, hemorrhage, chronic active gastritis and ulcer occurred at 48th week. The control group of gerbils without H.pylori colonization and histological lesions. Conclusion H.pylori infection causes the normal flora in Mongolian gerbils to change, resulting in gastritis and gastric ulcer.