目的 观察Earle’s液、新配最小必需培养基(MEM)及原 MEM对神经元活性的影响。方法 神经元培养 10 d后,分别换为Earle’s液、新配MEM及原MEM,12 h和 24 h后用乳酸脱氢酶(LDH)试剂盒测定细胞的 LDH漏出量,四甲基偶氮唑盐(MTT)微量酶反应比色法测定MTT代谢率。结果 神经元在不同培养介质中孵育 12 h和 24 h后,细胞 LDH的漏出量及 MTT代谢率有非常显著性差异( P <0.01)。结论 不同孵育液对神经元的活性有影响。 Objective To observe the effect of Earle’s solution, minimal essential medium (MEM) and original MEM on neuronal activity. Methods After cultured for 10 days, the neurons were respectively replaced by Earle’s fluid, newly-added MEM and original MEM, and the LDH leakage of cells was measured by lactate dehydrogenase (LDH) kit after 12 h and 24 h. Salt (MTT) micro-enzyme reaction colorimetric MTT metabolic rate. Results After neurons were incubated in different culture media for 12 h and 24 h, LDH leakage and MTT metabolic rate were significantly different (P <0.01). Conclusion Different incubation solutions have an impact on neuronal activity.