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目的 探讨端粒酶活性 (TA )和细胞周期蛋白依赖激酶抑制蛋白 p2 7kip1在肝癌中的表达及其相互关系。方法 采用端粒重复序列扩增 酶联免疫吸附 (TRAP ELISA)方法测定 2 7例肝细胞癌 (HCC)和 2 3例肝硬化组织中的端粒酶活性 ,采用免疫组织化学方法测定 p2 7kip1在HCC中的表达。结果 2 7例HCC中 2 4例端粒酶阳性 ,3例阴性 ;2 3例肝硬化中 3例阳性 ,2 0例阴性 ,两者差异有非常显著性 ( r =0 .484,P <0 .0 0 1) ;HCC中平均 p2 7kip1标记指数为 5 0 .30± 19.83;端粒酶活性与p2 7kip1呈正相关 (P <0 .0 5 )。结论 测定端粒酶活性有利于早期发现肝癌 ,端粒酶的激活与p2 7kip1蛋白表达有关。
Objective To investigate the expression of telomerase activity (TA) and cyclin-dependent kinase inhibitor p2 7kip1 in hepatocellular carcinoma and their relationship. Methods The telomerase activity in 27 cases of hepatocellular carcinoma (HCC) and 23 cases of cirrhosis were determined by telomere repeat amplification enzyme-linked immunosorbent assay (TRAP ELISA). The immunohistochemical method for the determination of p7 7kip1 was used. Expression in HCC. Results Twenty-four cases of HCC were positive for telomerase in 24 cases and negative in 3 cases; 3 cases were positive for cirrhosis and 3 cases were negative for cirrhosis, and the difference was significant (r=0.484, P<0). .0 0 1) The mean p2 7 kip1 labeling index in HCC was 5 0 .30 ± 19.83. The telomerase activity was positively correlated with p2 7kip1 (P < 0.05). Conclusion The determination of telomerase activity is beneficial to the early detection of liver cancer. The activation of telomerase is related to the expression of p2 7kip1 protein.