建立了单管定量检测食品中大肠菌群的快速检测系统。通过光子接收器采集样品在410 nm处吸光度变化数据,由固化在计算机内的程序来实现样品中大肠菌群的单管定量快速检测。结果表明,检测系统可检测出7 cfu/m L(g)样品,菌浓度在7~105cfu/m L(g)范围内具有良好的线性关系。当菌浓度为7~105cfu/m L(g)时所需检测时间为558~275 min,检测结果与国标法检测结果之间无显著差异。可满足常见食品样品大肠菌群的单管定量快速检测需求。 The establishment of a single tube quantitative detection of coliform bacteria in food rapid detection system. The absorbance data of the samples at 410 nm were collected by a photon receiver, and a single tube quantitative rapid test of the coliform bacteria in the sample was performed by a program solidified in the computer. The results showed that the detection system could detect 7 cfu / m L (g) samples with good linearity in the range of 7-105 cfu / m L (g). When the concentration of bacteria is 7 ~ 105cfu / m L (g), the required test time is 558 ~ 275 min, there is no significant difference between the test results and national standard test results. To meet the common food samples coliform bacteria single tube quantitative rapid detection needs.