论文部分内容阅读
作者从培养的可分泌人黑色素瘤特异性单克隆抗体的杂交瘤细胞Leo Mel3提取RNA,反转录成cDNA.用合成的寡核苷酸引物从中扩增了抗体的轩、重链可变区基因,并克隆入质粒.随机挑取阳性克隆各一个进行核苷酸序列分析.见所克隆基因确可编码小鼠抗体的轻、重链可变区。
The authors extracted RNA from Leo Mel3, a hybridoma that secreted human melanoma-specific monoclonal antibodies, and reverse transcribed into cDNA from which the Xuan, heavy chain variable region of the antibody was amplified using synthetic oligonucleotide primers Genes were cloned into plasmids and one of the positive clones was randomly selected for nucleotide sequence analysis, see the cloned gene indeed encoding the light and heavy chain variable regions of mouse antibodies.