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目的：检测外周血淋巴细胞部份的结核菌ＤＮＡ。方法：外周血标本取自１４０例志愿者，以葡聚糖－泛影葡甲胺密度梯度高心分离出淋巴细胞，抽提出的ＤＮＡ作ＰＣＲ扩增结核菌ＤＮＡ１２３ｂｐ，扩增产物行琼脂糖凝胶电泳检测。结果：新近诊断的活动性结核病人，９０．６％（９６／１０６）ＰＣＲ阳性，２例完成６ｍ短化治疗者也显示阳性结果，１例完成９ｍ治疗者、２例３年前治愈者、２９例健康对照者均为阴性；１４０例受检查的同份血标本，抽提出淋巴细胞后的血浆ＰＣＲ分析全部为阴性结果。结论：这种ＰＣＲ技术可用于结核病的快速诊断，尤其适用无痰病人。该结果也提示我们注意，淋巴细胞是否会直接吞噬和杀灭结核菌。
Objective: To detect the TB DNA of peripheral blood lymphocytes. Methods: Peripheral blood samples were obtained from 140 volunteers. Lymphocytes were isolated by high-density dextran-meglumine meglumine gradient centrifugation. DNA extracted from the extracted DNA was used to amplify 123bp DNA of M. tuberculosis. The amplified product was agarose gel Gel electrophoresis test. RESULTS: Among the newly diagnosed active tuberculosis patients, 90.6% (96/106) were positive for PCR, and 2 patients who completed 6m short treatment also showed positive results. One patient completed 9m treatment, two patients cured three years ago, Twenty-nine healthy controls were negative; all the 140 blood samples examined and plasma PCR analysis of lymphocytes extracted were all negative results. Conclusion: This PCR technique can be used for the rapid diagnosis of tuberculosis, especially for patients without sputum. This result also prompts us to note whether lymphocytes will directly engulf and kill Mycobacterium tuberculosis.