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目的探讨干细胞因子(stem cell factor,SCF)联合抗白细胞介素-17A抗体(anti-IL-17A)对小鼠脑缺血预后的影响。方法通过线栓法对C57BL/6小鼠建立大脑中动脉栓塞(middle cerebral artery occlusion,MCAO)模型,60 min后取出线栓,恢复右侧大脑中动脉血流,建立脑缺血再灌注模型。小鼠脑缺血再灌注后分别给予腹腔注射生理盐水(normal saline,NS)、anti-IL-17A、SCF、anti-IL-17A+SCF。观察各组小鼠MCAO术后脑梗死体积、脑组织病理损伤和脑水肿程度、神经功能评分、神经细胞凋亡和炎症反应等变化。结果与NS组相比,其余各治疗组脑梗死体积、脑组织病理损伤和脑水肿均显著减轻(P<0.05),神经功能评分得到明显改善(P<0.05),神经细胞凋亡减少(P<0.05),且antiIL-17A+SCF组显著优于anti-IL-17A组和SCF组(P<0.05)。此外,anti-IL-17A还能明显减少脑组织IL-17A和IL-1β的表达(P<0.05)。结论 SCF联合anti-IL-17A能更好地改善脑缺血损伤和促进神经功能恢复。
Objective To investigate the effect of stem cell factor (SCF) combined with anti-IL-17A antibody on the prognosis of cerebral ischemia in mice. Methods The middle cerebral artery occlusion (MCAO) model of C57BL / 6 mice was established by thread plug method. After 60 min, the thrombus was removed and the right middle cerebral artery blood flow was restored. The cerebral ischemia-reperfusion model was established. The mice were injected intraperitoneally with normal saline (NS), anti-IL-17A, SCF and anti-IL-17A + SCF respectively after cerebral ischemia and reperfusion. The volume of cerebral infarction, pathological damage and degree of brain edema, nerve function score, neuron apoptosis and inflammatory reaction in MCAO group were observed. Results Compared with NS group, the volume of cerebral infarction, the pathological damage of brain tissue and brain edema were significantly decreased (P <0.05), the scores of neurological function were significantly improved (P <0.05), and the apoptosis of neurons was decreased <0.05), and antiIL-17A + SCF group was significantly better than anti-IL-17A group and SCF group (P <0.05). In addition, anti-IL-17A also significantly reduced the expression of IL-17A and IL-1β in brain tissue (P <0.05). Conclusion SCF combined with anti-IL-17A can better improve cerebral ischemic injury and promote neurological recovery.