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目的 :探讨 p16基因的异常表达与原发性肝癌发生、发展的关系。方法 :应用PCR、SSCP分析及DNA序列测定技术研究了 30例原发性肝癌组织中的 p16基因外显子 2 (E2 )的缺损情况。结果 :( 1)p16基因缺损的检出率与肝癌的肿瘤组织类型无关 ,在肝细胞癌与胆管细胞癌中p16基因缺损的检出率分别为 33 .33 %和 2 0 .0 0 % ,两组间无显著差异 (P >0 .0 5 ) ;( 2 ) p16基因缺损率的高低与组织学分级密切相关 (P <0 .0 5 )。结论 :结果提示p16基因的缺损与原发性肝癌的组织学分级相关 ,它可能参与原发性肝癌的发生、发展过程
Objective: To investigate the relationship between the abnormal expression of p16 gene and the occurrence and development of primary liver cancer. METHODS: The exon 2 (E2) defect of p16 gene in 30 cases of primary hepatocellular carcinoma was studied by PCR, SSCP analysis and DNA sequencing. Results: (1) The detection rate of p16 gene deletion was not related to the tumor tissue type of HCC. The detection rate of p16 gene deletion in hepatocellular carcinoma and cholangiocellular carcinoma was 33.33% and 20.0%, respectively. There was no significant difference between the two groups (P > 0.05); (2) The defect rate of p16 gene was closely related to histological grade (P < 0.05). Conclusion: The results suggest that the defect of p16 gene is related to the histological grade of primary liver cancer. It may be involved in the occurrence and development of primary liver cancer.