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目的研究人源抗人免疫缺陷病毒１型（ＨＩＶ－１）ｇｐ１２０单链抗体（ＳｃＦｖ）。方法以人工合成的ＨＩＶ－ｌｇｐ１２０Ｖ３环多肽为抗原，利用噬菌体抗体库技术，筛选含有抗－ｇｐ１２０ＳｃＦｖ基因的噬菌体，提取质粒，转化大肠杆菌ＨＢ２１５１，表达可溶性ＳｃＦｖ。结果经ＳＤＳ－ＰＡＧＥ和Ｗｅｓｔｅｒｎｂｌｏｔ分析，表达产物分子量为２８ｋＤ左右，且具有ｃ－ｍｙｃ活性；ＥＬＩＳＡ和Ｄｏｔｂｌｏｔ结果表明，可溶性ＳｃＦｖ具有较好的抗原结合活性和较强的特异性；竞争性ＥＬＩＳＡ实验结果进一步证明表达产物的特异抗－ｇｐ１２０活性。结论该技术便捷有效，可大量获得人源抗ＨＩＶ抗体片段，为进一步研究抗ＨＩＶ抗体的生物活性和ＨＩＶ感染诊治打下基础
Objective To investigate human anti-human immunodeficiency virus type 1 (HIV-1) gp120 single chain antibody (ScFv). Methods The phage antibody library was used to screen for the phage containing the anti-gp120ScFv gene. The plasmid was extracted and transformed into E. coli HB2151 to express soluble ScFv. Results SDS-PAGE and Western blot analysis showed that the expressed product had a molecular weight of about 28kD and had c-myc activity. ELISA and Dotblot showed that soluble ScFv had good antigen-binding activity and strong specificity. The results of competitive ELISA Further demonstrating the specific anti-gp120 activity of the expression product. Conclusion The technique is convenient and effective and can obtain a large number of human anti-HIV antibody fragments, laying a foundation for further research on the biological activity of anti-HIV antibodies and diagnosis and treatment of HIV infection