用新鲜尸体墨汁福尔马林混合液灌注,将腹直肌皮瓣由上至下平均分为6个区,分别取材、流水冲洗、10%福尔马林固定、石蜡包埋、连续切片、伊红染色。在光镜下观察皮瓣内微血管分为乳头层、乳头下层、真皮深层、浅筋膜层及深筋膜层。 The fresh cadaveric instillation of formalin was used to perfuse the rectus abdominis flaps. The flaps of the rectus abdominis muscle were evenly divided into 6 zones from top to bottom. The flap was washed with water, washed with 10% formalin, embedded in paraffin, Eosin staining. Under light microscope, the capillaries in the flap were divided into the papillary layer, the inferior layer of the nipple, the deep dermis, the superficial fascia and the deep fascia.