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要研究一种植物病毒的性质和有效地防治病毒病害,都需要进行植物病毒的分离和提纯。病毒的分离提纯技术,就是利用病毒与寄主细胞组分的差异,尽可能地从寄主物质中制备得高纯度并具感染性的病毒粒子制剂。病毒表面均含有蛋白质外壳(有些还含有脂质外膜),因此一些提纯蛋白质的技术也可用于病毒的提纯。一、选择合适的寄主植物材料首先,寄主植物要含有足够浓度的病毒。一般认为每公斤鲜叶材料至少应含有5～10毫克干病毒或更多的含量。一种植物病毒往往有多种寄主植物,它在不同的寄主植物中含量可以有很大差异。最好用病毒含量高、寄主植物成分容易除去的材料抽提和纯化。病毒最好从系统感染的寄主中提取,因为在这些植株中病毒浓度较高。新近发病显现症状的幼嫩植株,一般要比老的、久经感染的植株具有更高的病毒含量。但是,这并非普遍规律。如中国大白菜
To study the nature of a plant virus and effective control of virus diseases, the need for plant virus isolation and purification. Virus isolation and purification technology, is to use the difference between the virus and the host cell components, as far as possible from the host material preparation of high purity and infectivity of the virus particle preparation. The virus surface contains protein shells (some also contain lipid adventitia), so some protein purification techniques can also be used for virus purification. First, select the appropriate host plant material First, the host plant to contain enough concentration of the virus. It is generally believed that fresh leaf material per kg should contain at least 5 to 10 mg of dry virus or more. A plant virus often has multiple host plants, which can vary widely in host plants. It is best to use high virus content, the host plant ingredients easily removed material extraction and purification. It is best to extract the virus from the host that is infected by the system because of the high virus concentration in these plants. Young plants that have recently developed symptoms appear to have a higher virus content than older, well-established plants. However, this is not a universal law. Such as Chinese cabbage