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为了快速创制大麦赤霉病抗性存在差异的加倍单倍体(DH)株系,采用单花滴注法和喷雾法对诱变结合小孢子培养的379份大麦DH株系和原始品种花30的赤霉病抗扩展性和抗侵染性进行评价,同时对获得的抗性突变体材料和花30进行赤霉菌诱导下的大麦病程相关基因非表达子(NPR1、NPR2、NPR3)、茉莉酸介导的植物防御的调控因子(JAV1)以及乙烯响应因子(ERF1)的基因表达分析。结果表明,通过连续2年大田人工接种鉴定,从中获得了5份抗扩展性和抗侵染性均优于花30的候选株系,9份抗扩展性和抗侵染性均劣于花30的候选株系;赤霉病抗性突变体A1-190与原始品种花30在上述基因表达上存在明显差异,推测这些基因的表达与其抗病性的变化相关。研究结果表明诱变结合小孢子培养可以创制的DH株系在大麦赤霉病抗性存在差异。本研究结果为大麦抗赤霉病基因定位克隆及分子育种奠定了材料基础。
In order to rapidly establish doubled haploid (DH) lines with different resistances to FHB, 379 barley DH lines induced by mutagenesis and microspore culture and 30 Sclerotinia sclerotiorum, the resistance to scab and Sclerotinia sclerotiorum were evaluated. At the same time, the resistant mutants and flowers 30 were screened for the non-expression of barley disease-related genes (NPR1, NPR2, NPR3), jasmonic acid Mediated regulation of plant defense (JAV1) and gene expression analysis of the ethylene response factor (ERF1). The results showed that, by two years of field artificial inoculation identification obtained from the 5 strains of both anti-expandability and anti-infectivity are better than 30, 9 anti-expansive and anti-infective are inferior to spend 30 . The results showed that there was a significant difference in the above gene expression between the scleroderma resistant mutant A1-190 and the original cultivar Hua30. It is speculated that the expression of these genes is related to the change of their disease resistance. The results showed that there was a difference in the resistance of the DH strain to the DH strain that could be created by mutagenesis and microspore culture. The results of this study laid the material foundation for the positional cloning and molecular breeding of barley blast resistance genes.