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在培养的肺动脉平滑肌细胞（ＰＡＳＭＣ）内加入青心酮（３．４－ＤＨＡＰ）（６．２５×１０－５Ｍ－１０－３Ｍ／Ｌ）注射液，用结晶紫染色法，３Ｈ－ＴｄＲ掺入以及用ＳＰ免疫细胞化学检测ＰＣＮＡ表达，观察对肺动脉平滑肌细胞增殖的影响，结果表明青心酮对１０％小牛血清引起的ＰＡＳＭＣ增生的作用与异搏定（６．２５×１０－６－１０－４Ｍ／Ｌ）相似，呈剂量依赖性抑制细胞增殖。提示青心酮可用于慢性肺动脉高压肺血管改建的防治。
Chlorohexanone (3.4-DHAP) (6.25×10-5M-10-3M/L) injection was added to cultured pulmonary artery smooth muscle cells (PASMC), and 3H-TdR was incorporated by crystal violet staining. And the expression of PCNA was detected by SP immunocytochemistry, and the effect on the proliferation of pulmonary artery smooth muscle cells was observed. The results showed that the effect of Qingxin Ketone on the proliferation of PASMC induced by 10% calf serum was verapamil (6.25×10-6-10). -4M/L) similarly inhibited cell proliferation in a dose-dependent manner. It is suggested that Qingxin Ketone can be used for prevention and treatment of pulmonary hypertension in chronic pulmonary hypertension.