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目的研究星虫多糖(Sipunculus nudus L.polysaccharide,SNP)对亚急性辐射损伤小鼠造血系统的保护作用。方法选用雄性BALB/c,采用~(137)Csγ射线对小鼠进行1次性全身照射(总剂量为4.0Gy),建立亚急性辐射损伤模型。照射前连续灌胃给药7d,照射后继续给药14d。用细胞分析仪测定外周血象,流式细胞仪检测骨髓造血干细胞含量与细胞凋亡率。照射后24h取小鼠股骨进行HE染色观察骨髓病理变化。结果经~(137)Csγ射线照射后,与辐照模型组相比,照后第10天,90mg/kg SNP小鼠外周血象红细胞数量显著提高(P<0.05),SNP 3个剂量组血小板数量均显著提高(P<0.05);照后第14天,270mg/kg SNP组小鼠脾脏指数与30mg/kg SNP组小鼠睾丸指数显著提高(P<0.05),30mg/kg SNP组骨髓造血干细胞数量显著提高(P<0.05),SNP各给药组骨髓细胞凋亡率显著下降(P<0.01),骨髓组织中有核细胞数量显著增多。结论SNP对辐射损伤小鼠的造血系统具有较好的保护效果。
Objective To study the protective effect of Sipunculus nudus L. polysaccharide (SNP) on hematopoietic system in subacute radiation-induced mice. METHODS: Male BALB/c mice were used to perform whole body irradiation with a total dose of 4.0 Gy using ~(137)Cs γ-rays to establish a subacute radiation injury model. The rats were administered intragastrically for 7 days before irradiation and continued for 14 days after irradiation. The peripheral blood was measured with a cell analyzer, and the hematopoietic stem cell content and apoptosis rate were measured by flow cytometry. Twenty-four hours after irradiation, the femurs of the mice were taken for HE staining to observe the pathological changes of the bone marrow. Results After irradiation with ~(137)Csγ-irradiation, compared with the irradiated model group, the number of peripheral blood red blood cells in 90 mg/kg SNP mice was significantly increased on the 10th day after irradiation (P<0.05). Significant increase (P<0.05); On the 14th day after irradiation, spleen index of 270 mg/kg SNP mice group and 30 mg/kg SNP group mice testicle index increased significantly (P<0.05), 30 mg/kg SNP group bone marrow stem cells The number was significantly increased (P<0.05). The apoptosis rate of bone marrow cells in each group of SNP significantly decreased (P<0.01), and the number of nucleated cells in the bone marrow tissue increased significantly. Conclusion SNP has a good protective effect on the hematopoietic system of radiation-injured mice.