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目的探讨局灶性脑缺血再灌注后大鼠脑组织缺氧诱导因子-1α(hypoxia inducible factor-1α,HIF-1α)的表达及意义。方法制备大鼠局灶性脑缺血再灌注模型,动物分为缺血2 h再灌注6 h、1、2、3、4 d及假手术对照组。应用免疫组化法检测HIF-1α蛋白、原位杂交及RT-PCR法检测HIF-1α mRNA的表达变化。结果免疫组化及原位杂交法检测显示:大鼠缺血再灌注6 h,缺血侧脑组织梗死灶周边HIF-1α及HIF-1α mRNA阳性反应已出现,随再灌注时间的延长阳性表达增强,一直持续到第3天,第4天显著下降;RT-PCR检测HIF-1α mRNA在6 h后已有表达,随再灌注时间的延长,HIF-1α mRNA的表达呈上升的趋势,在第3天达高峰,第4天显著下降。结论脑缺血再灌注损伤可以诱导HIF-1α表达增强,提示HIF-1α对缺血性脑损伤有保护作用。
Objective To investigate the expression and significance of hypoxia inducible factor-1α (HIF-1α) in rat brain after focal cerebral ischemia and reperfusion. Methods Focal cerebral ischemia-reperfusion model was established in rats. Animals were divided into 6 h, 1, 2, 3 and 4 d after reperfusion and sham operation control group. HIF-1αprotein was detected by immunohistochemistry, the expression of HIF-1α mRNA was detected by in situ hybridization and RT-PCR. Results The results of immunohistochemistry and in situ hybridization showed that the positive reaction of HIF-1α and HIF-1α mRNA in the ischemic brain tissue around infarct had been observed 6 h after ischemia-reperfusion in rats, and was positively correlated with the reperfusion time Increased significantly until day 3, and decreased significantly on day 4. The expression of HIF-1α mRNA was detected after 6 h by RT-PCR. The expression of HIF-1α mRNA increased with the time of reperfusion, The third day reached its peak, the fourth day decreased significantly. Conclusions Cerebral ischemia-reperfusion injury can induce the expression of HIF-1α to be enhanced, suggesting that HIF-1α may have a protective effect on ischemic brain injury.