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目的:建立高效液相色谱法测定壮腰健肾片中槲皮素含量的方法。方法:采取HPLC法VP-ODS柱(150mm×4.6mm,5μm);甲醇-0.4%磷酸溶液(85∶15);检测波长:370nm;流速:1.0mL.min-1;温度:室温。结果:槲皮素检测浓度在1.02～10.20μg.mL-1(r=0.9998)范围内与峰面积呈良好的线性关系,平均加样回收率为99.1%,RSD=1.9。结论:本法具有操作简单方便、灵敏、准确率高的特点,可用于壮腰健肾片的质量检测。
Objective: To establish a method for the determination of quercetin in Zhuangyaojianxuan Tablet by HPLC. Methods: HPLC-VP-ODS column (150mm × 4.6mm, 5μm); methanol-0.4% phosphoric acid solution (85:15); detection wavelength: 370nm; flow rate: 1.0mL.min-1; Results: The calibration curve of quercetin showed a good linear relationship with the peak area in the range of 1.02-10.20μg.mL-1 (r = 0.9998). The average recovery was 99.1% and RSD was 1.9. Conclusion: This method has the characteristics of simple and convenient operation, sensitive and high accuracy, which can be used for the quality inspection of Zhuangyao Jian Shen Tablet.