论文部分内容阅读
为了研究甜樱桃(Prunus avium)成花调控关键基因在不同砧穗组合中表达差异,以甜樱桃‘艳阳’(Sunburst)为接穗,‘ZY-1’(P.cerasus)、马哈利(P.mahaleb)和‘兰丁2号’(P.avium × P.pseudocerasus)为砧木的嫁接组合为试材,调查统计4年干龄不同砧穗组合的总花芽量及开花情况;克隆甜樱桃成花调控网络中EARLY FLOWERING 3(ELF3)、CONSTANS(CO)、FLOWERING LOCUS T(FT)等9个关键作用基因,并利用实时荧光定量PCR对不同砧穗组合接穗的幼叶、叶芽、成龄叶、花芽、叶芽附近叶片叶柄及花芽附近叶片叶柄中这些基因的表达量鉴定。结果显示,艳阳/ZY-1、艳阳/马哈利和艳阳/兰丁2号组合的平均单株花芽量分别为279、288和317朵,不存在明显差异,但不同组合的花期却明显不同,当艳阳/马哈利有72%花芽处于开放期时,艳阳/兰丁2号的花开放比例只有7%,艳阳/ZY-1的花开放比例为49%;此外,PaELF3、PaCO、PaFT等9个成花关键基因,在同一时期不同砧穗组合的接穗组织中表达量存在差异,其中PaAP1在不同砧穗组合的花芽中表达模式与花期规律一致,表明PaAP1与樱桃花期调控密切相关。
In order to study the expression differences of key genes of flower bud control in sweet cherry (Prunus avium) in different combinations of scion heads, we used Sunburst (sweet cherry) as scion, ’ZY-1’ .mahaleb and P.avium × P.pseudocerasus were selected as the grafting materials for the rootstocks. The total amount of flower buds and flowering of different combinations of scion heads with 4-year-old dry age were investigated. Nine key genes such as EARLY FLOWERING 3 (ELF3), CONSTANS (CO) and FLOWERING LOCUS T (FT) were regulated by flow cytometry. Fluorescent quantitative PCR was performed on young leaves, leaf buds, , Flower buds, leaf buds near the leaf petiole and flower buds near the petiole in the expression of these genes were identified. The results showed that the average flower buds per plant of YanYang / ZY-1, YanYang / Mahali and YanYang / Landing No.2 were 279, 288 and 317, respectively. There was no significant difference but the flowering of different combinations was obviously different, When 72% of the flower buds of Yanyang / Mahali are in the open, the bloom ratio of Yanyang / Landing 2 is only 7%, and the ratio of the open sun / ZY-1 flower is 49%. In addition, PaELF3, PaCO, PaFT, etc. The expression of 9 key genes of flower formation in scion of different combinations of scion were different during the same period. Among them, the expression pattern of PaAP1 in flower bud of different combinations of scion was consistent with that of flowering, indicating that PaAP1 is closely related to the flowering regulation of cherry.