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目的 研究野生型p5 3基因对人胶质瘤细胞生长及放疗敏感性的影响。方法 将野生型 p5 3基因导入U 2 5 1细胞 ,通过逆转录 聚合酶链反应 (RT PCR)检测 p5 3基因的表达。U2 5 1细胞分为 4组 :对照组、转染组、放疗组、转染联合放疗组。用四甲基偶氮唑盐微量酶反应比色法(MTT法 )和流式细胞仪检测p5 3基因或 /和放疗 (3、6、9Gy)对U 2 5 1细胞生长抑制及凋亡的影响。结果 通过RT PCR证实了 p5 3基因在U 2 5 1细胞中的表达。MTT检测发现 p5 3基因对U2 5 1细胞的生长抑制率为 (79.60± 5 .69) %。当放射剂量为 3、6、9Gy时 ,U2 5 1细胞的生长抑制率分别为(17.0 6± 4.3 5 ) % ,(17.3 9± 1.67) % ,(18.73± 4.68) %。当 p5 3基因与放疗 (3、6、9Gy)联合作用时 ,抑制率分别为 (80 .60± 5 .3 5 ) % ,(90 .3 0± 1.67) % ,(91.3 0± 2 .0 1) %。p5 3基因转染所产生的U 2 5 1细胞凋亡率为 17.3 8%。放疗 (3、6、9Gy)引起的细胞凋亡率分别为 4.61% ,4.84% ,5 .40 %。当p5 3基因与放疗 (3、6、9Gy)联合作用时 ,凋亡率分别为17.80 % ,2 0 .0 3 % ,2 2 .3 4%。结论 野生型p5 3基因与放疗对人胶质瘤细胞生长有协同抑制作用
Objective To investigate the effect of wild type p53 gene on the growth and radiosensitivity of human glioma cells. Methods Wild type p5 3 gene was introduced into U 2 51 cells and the expression of p5 3 gene was detected by reverse transcriptase polymerase chain reaction (RT PCR). U2 5 1 cells were divided into 4 groups: control group, transfection group, radiotherapy group, transfection combined with radiotherapy group. The inhibitory effects of p5 3 gene and / or radiotherapy (3, 6, 9 Gy) on U 2 51 cell proliferation and apoptosis were detected by MTT assay and flow cytometry influences. Results The expression of p5 3 gene in U 2 51 cells was confirmed by RT PCR. The inhibition rate of p5 3 gene to U2 5 1 cells was (79.60 ± 5.69)% by MTT assay. The growth inhibition rate of U2 5 1 cells were (17.0 ± 4.3 5)%, (17.3 9 ± 1.67)% and (18.73 ± 4.68)% respectively when the radiation doses were 3, 6 and 9 Gy. When combined with radiotherapy (3, 6, 9 Gy), the inhibitory rates of p5 3 gene were (80.60 ± 5.53)%, (90.3 ± 1.67)%, (91.3 0 ± 2.0) 1) %. The apoptosis rate of U 2 5 1 cells induced by p5 3 gene transfection was 17.3 8%. The rates of apoptosis induced by radiotherapy (3, 6 and 9 Gy) were 4.61%, 4.84% and 5.40%, respectively. When the combination of p5 3 gene and radiotherapy (3, 6, 9 Gy), the apoptotic rates were 17.80%, 2.03% and 22.34% respectively. Conclusion Wild-type p53 gene and radiotherapy have a synergistic inhibitory effect on the growth of human glioma cells