目的 建立人脑脊液中利多卡因的高效液相色谱(HPLC)分析方法。方法 以空白人脑脊液添加标准利多卡因进行HPLC分析,并系统考察实验中的色谱条件、样品处理方法、线性关系、精密度及回收率。结果 所建方法的线性范围是1.0～20.0μg·ml-1(r=0.9993),最低检测浓度为1.0μg·ml-1(S/N≥3),日内、日间检测的相对标准偏差≤3.0％,回收率为98.3％～102.7％。结论 所建方法定量检测人脑脊液中的利多卡,灵敏、准确、快速。 Objective To establish a high performance liquid chromatography (HPLC) method for the determination of lidocaine in human cerebrospinal fluid. Methods Standard lidocaine was added to human cerebrospinal fluid (CSF) for HPLC analysis. The chromatographic conditions, sample handling, linearity, precision and recovery were systematically investigated. Results The linear range of the method was 1.0-20.0μg · ml-1 (r = 0.9993), the lowest detection concentration was 1.0μg · ml-1 (S / N≥3). The relative standard deviations 3.0%, the recovery rate was 98.3% ~ 102.7%. Conclusion The established method for the quantitative detection of lidocaine in human cerebrospinal fluid is sensitive, accurate and rapid.