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为建立一种检测幽门螺杆菌感染的最佳方法，用尿苷酶抗污染聚合酶链反应（改良式ＰＣＲ）检测幽门螺杆菌，并与组织病理学检查、尿素酶试验、酶联免疫吸附试验及细菌培养相比较。共９５例病人５种方法的检出率分别为８３．１％，７８．９％，７０．５％，６６．３％，４２．１％。２项检查阳性即判断为阳性，否则为阴性。９５例病人中有７９例阳性，１６例阴性。ＰＣＲ方法的敏感性、特异性、阳性预测值及阴性预测值分别为：９７．５％，８７．５％，９７．５％，８７．５％，明显优于其它检查方法。改良后的ＰＣＲ方法为其从单纯的科研方法转为临床应用作了尝试。
In order to establish a method to detect H. pylori infection, H. pylori was detected by uridinease anti-pollution polymerase chain reaction (modified PCR) and compared with histopathological examination, urease test, enzyme-linked immunosorbent assay And bacterial culture compared. A total of 95 cases of the five detection methods were 83.1%, 78.9%, 70.5%, 66.3%, 42.1%. 2 positive test that is judged as positive, otherwise negative. Of the 95 patients, 79 were positive and 16 were negative. The sensitivity, specificity, positive predictive value and negative predictive value of PCR method were 97.5%, 87.5%, 97.5% and 87.5% respectively, which was significantly better than other methods. The improved PCR method for its transfer from a simple scientific method to clinical application made an attempt.