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牛磺酸(Tau)是哺乳动物中枢神经系统中的一种神经介质,含量较高,与各种实验性癫痫发作有关,并能抑制离体脑组织中神经原的活性,因此测定Tau含量变化,以了解脑神经原的作用机制已日趋重要,本文介绍一种较前报道的更为快速简便的高压液相色谱-电化学检测分析方法,联用邻苯二醛(OPA)/β巯基乙醇(BME)衍生物法,可在2min内测定小鼠脑组织(包括纹状体、下丘脑、海马和大脑皮等)中Tau的含量。作者将Tau、y-氨基丁酸(GABA)
Taurine (Tau) is a neurotransmitter in the mammalian central nervous system that is associated with a wide range of experimental seizures and inhibits the activity of neurons in isolated brain tissue, thus measuring changes in Tau content In order to understand the mechanism of action of brain neurons has become more and more important. In this paper, a more rapid and simple method of high pressure liquid chromatography-electrochemical detection and detection, which is reported previously, is introduced in this paper. Combined with OPA / β-mercaptoethanol (BME) derivative method, the content of Tau in mouse brain tissue (including striatum, hypothalamus, hippocampus and cerebral cortex, etc.) can be measured in 2 minutes. The authors will Tau, y-aminobutyric acid (GABA)