建立测定微乳中水飞蓟素含量的反相高效液相色谱法.采用ODS C18色谱柱,检测波长为288 nm,流动相为甲醇-水-冰醋酸(42:58:0.5,v/v/v),流速为1 mL/min.水飞蓟宾标准曲线的线性范围为10-1000μg/mL,平均回收率为99.0%-100.7%,日内和日间精密度均低于1.6%.该法简单、快速、精确、准确,可用于水飞蓟素微乳制剂质量控制要求.“,”We established a RP-HPLC method for the analysis of silymarin in microemulsion. Silymarin was separated using a ODS Cis column and monitored at the wavelength 288 nm. The mobile phase consisting of methanol-water-acetic acid (42:58:0.5, v/v/v) was pumped at a flow rate of 1.0 mL/min. The linear range of calibration curve was 10-1000 μg/mL. The average recovery was 99.0%-100.7% for silybin isomers. The RSD values of inter-day and intra-day assays were lower than 1.6% for silybin isomers. The method is simple, rapid, reproducible and precise for the quantitative determination of silymarin in microemulsion.