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目的：探讨基因工程藻高效杀蚊幼活性，以确定其质粒表达情况。方法：抗性筛选及蚊虫毒效测试。结果：随机筛选出的基因工程单藻落无一抗性丢失，且３种基因工程藻对淡色库蚊仍具较高毒效，ＬＣ５０（２４小时）Ａ．７１２０ｐＤＣ２６为８．２６×１０３ｃｅｌｓ／ｍｌ；Ａ．ｓｕｂｔｒｏｐｉｃｐＤＣ２６为２．６２×１０４ｃｅｌｓ／ｍｌ；Ａ．ｃｙｌｉｎｄｒｉｃａｔｏｘ为４．２１×１０３ｃｅｌｓ／ｍｌ。结论：穿梭质粒在基因工程藻细胞内稳定遗传，并持续高效表达。
Objective: To investigate the highly efficient mosquito-killing activity of genetically engineered algae in order to confirm the expression of its plasmid. Methods: Resistance screening and mosquito toxicity test. Results: No genetically engineered single algae were randomly selected, and the three genetic engineering algae were still highly toxic to Culex. (LC50 (24 hours)). 7120pDC26 is 8.26 × 103cels / ml; subtropicpDC26 is 2.62 × 104cels / ml; The cylindricatox is 4.21 x 103cels / ml. Conclusion: The shuttle plasmid is stably inherited in the genetically engineered algal cells and expressed continuously and efficiently.