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目前,尚缺乏可以对细胞凋亡进行普遍的生化检测的或抗原的标志物。现有的检测方法有: 1 形态学鉴定 不同类型和种系的细胞在电镜或光镜下表现出的形态特征非常近似,是最无争议的证据。但因凋亡的发生和消失十分迅速,某一时间点存在的凋亡细胞数很少,检出率很低。 2 凝胶电泳 呈阶梯带型,具特征性。阶梯型凝胶电泳带是细胞凋亡的生化标志,由于核微体间DNA裂解所致。但随机DNA裂解发生在细胞坏死的后期,DNA破坏的残余片段不可能有恒定的大小,凝胶电泳时只能成为一片模糊的涂抹。而且与染色质
Currently, there is a lack of universal biochemical detection of apoptosis or antigen markers. The existing detection methods are as follows: 1 Morphological identification The morphological characteristics of different types and germ line cells under electron microscope or light microscope are very similar, which is the most uncontroversial evidence. However, due to the occurrence and disappearance of apoptosis is very rapid, the number of apoptotic cells present at a certain time is very small, and the detection rate is very low. 2 gel electrophoresis showed a ladder-type, characteristic. The ladder-type gel electrophoresis band is a biochemical marker of apoptosis due to nuclear DNA cleavage. However, random DNA cleavage occurs in the late stages of cell necrosis. The residual fragment of DNA damage can not be of a constant size and can only become a blurry smear on gel electrophoresis. And with chromatin