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目的 :研究大鼠海马结构内突触小泡蛋白年龄变化的影响。方法 :实验用 Wistar大鼠、免疫组织化学结合图像分析法。结果 :2 4月龄大鼠齿状回多形层、海马 CA3辐射层内突触小泡蛋白免疫反应产物的灰度值明显大于 18月龄鼠 ( P<0 .0 0 1,P<0 .0 0 5) ;12月龄鼠经 6和 12个月服用龟龄集后 ,齿状回多形层、海马 CA3辐射层内突触小泡蛋白免疫反应产物的灰度值均比同龄对照组显著降低 ( P<0 .0 5)。结论 :大鼠海马结构内突触小泡蛋白随着年龄的增加而减少 ;服用龟龄集可延缓神经元的衰老 ,防止海马结构内突触小泡蛋白的丢失 ,改善学习记忆功能
Objective: To study the effect of changes in the age of synaptic vesicles in rat hippocampal formation. Methods : Wistar rats were used in the experiment and immunohistochemistry combined with image analysis. RESULTS: The gray value of synaptic vesicular protein immunoreactivity in the dentate gyrus polymorphonal layer and hippocampal CA3 radiation layer was significantly greater in rats aged 4 months than in 18-month old rats (P<0.01, P<0). .0 0 5) Gray-level values of synaptic vesicle protein immunoreactive products in the dentate gyrus polymorphs and hippocampal CA3 radiation layer in 12-month-old rats after 6 and 12 months of age were compared with those of the same age controls. The group was significantly lower (P < 0.05). Conclusion: The synaptic vesicle protein in the rat hippocampal formation decreases with age; taking Guilingji can delay the aging of neurons, prevent the loss of synaptic vesicle protein in hippocampal formation, and improve learning and memory function.