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以闪金万年青、太阳万年青、粉黛万年青为对象进行了组培苗再生体系建立研究 ,结果表明 :这 3个品种万年青不定芽第一次分化所需的条件是 :MS+BA 3.0 mg/ L +NAA 0～0 .5mg/ L;丛生芽继代所需的培养条件是 MS+BA 1～ 2 mg/ L +NAA 0 .0 2～ 0 .5mg/ L ;芽年增殖系数为 5.3× 10 5～ 5.1× 10 7。组培苗生根所用激素为 IBA,使用浓度范围为 0 .5～ 1.0 mg/ L ,生根率 95%以上。培养基中 30 0～ 50 0 mg/ L浓度抗菌素的添加对细菌污染有明显的抑制作用。
The establishment of regeneration system of tissue culture seedlings was studied in the areas of flash gold evergreen, sun evergreen and indigo evergreen. The results showed that the conditions required for the first differentiation of adventitious buds of these three varieties were: MS + BA 3.0 mg / L + NAA 0 ~ 0.5mg / L. The culture conditions required for cluster generation were MS + BA 1 ~ 2 mg / L + NAA 0.2 ~ 0.5 mg / L, and the multiplication coefficient of shoots was 5.3 × 10 5 ~ 5.1 × 10 7. The rooting hormone used in tissue culture was IBA, the concentration range was 0.5 ~ 1.0 mg / L, and the rooting rate was above 95%. The addition of antibiotics at a concentration of 30 0 ~ 50 0 mg / L in the medium significantly inhibited bacterial contamination.