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去壳的水稻种子，当浸渍在２５℃蒸馏水中至４、１４、３６个小时，分别转移到小麦ＤＮＡ溶液内（５００ｍｇ／ｍｌ），施以负压（－０．０６Ｍｐａ）继续浸渍４小时。在同样温度下催芽，做根尖细胞学观察和幼芽同工酶分析。并测定ＤＮＡ浸种后的减少量。结果表明：浸渍４、１４、３６小时后外源ＤＮＡ减压转导处理的水稻种子，对外源ＤＮＡ的吸收量依次降低；细胞染色体数目和形状无明显变化；居中者同工酶差异明显，苗期即出现性状变异。减压渗透法将外源ＤＮＡ导入水稻种子的最佳时期为种子吸水第二阶段的前、中期。
Husked rice seeds were immersed in distilled water at 25 ° C for 4, 14, and 36 hours, respectively, and transferred to wheat DNA solution (500 mg / ml) for further 4 hours under negative pressure (-0.06 Mpa). In the same temperature germination, root tip cytology and shoots isozyme analysis. The amount of DNA after soaking was measured. The results showed that after 4, 14 and 36 hours of immersion, the amount of exogenous DNA absorbed by the foreign seeds reduced successively, while the number and shape of the chromosomes did not change obviously. Period that there is variation in traits. The best stage of introduction of exogenous DNA into rice seeds by pressure-reducing infiltration is the first and middle stage of the second stage of seed water absorption.