论文部分内容阅读
为探讨载脂蛋白ＡＩ－ＣⅢ基因区域ＤＮＡ多态性与动脉硬化性脑梗塞的关系，作者利用重组ＤＮＡ技术，对５９名正常血脂汉族个体和４４名动脉硬化性脑梗塞（ＡＢＩ）患者载脂蛋白ＡＩ－ＣⅢ基因区域ＤＮＡ多态频率和特征进行了分析，发现Ｓ２和Ｍ２２种多态性片段，正常人群等位基因频率为０．１５７和０．１９５，而在ＡＢＩ患者中分别为０．３６４和０．４８９．经统计学处理两组有显著性差异，提示Ｓ２和Ｍ２与ＡＢＩ发生有关。单体型分析表明，动脉硬化性脑梗塞患者Ｓ１－Ｍ２单体型频率（０．１２５）明显高于正常血脂人（０．０２９），两组有显著统计学差异，提示Ｓ１－Ｍ２单体型可作为一种遗传标记，有可能在中国人群中预测某些与脂类代谢障碍有关的疾病。
In order to investigate the relationship between apolipoprotein AI-CⅢ genomic DNA polymorphism and arteriosclerotic cerebral infarction, we used recombinant DNA technology to study the relationship between the apolipoprotein AI-CⅢ gene polymorphism and cerebral infarction in 59 patients with normal serum lipids and 44 patients with arteriosclerotic cerebral infarction The frequency and characterization of DNA polymorphism in AI-CⅢgene were analyzed. The allele frequencies of S2 and M22 polymorphism fragments in normal population were 0.157 and 0.195, respectively, while they were 0 in ABI patients. 364 and 0.489. Statistically treated two groups had significant differences, suggesting that S2 and M2 and ABI related. The haplotype analysis showed that the S1-M2 haplotype (0.125) in patients with arteriosclerotic cerebral infarction was significantly higher than that of normal lipids (0.029), the two groups had significant statistical difference, suggesting that S1-M2 monomer As a genetic marker, it is possible to predict certain diseases associated with dyslipidemia in the Chinese population.