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2013年6月四川省眉山市发生一起以发热、乏力、头痛、腹泻为主要症状的暴发疫情。为快速诊断致病因子,查找污染来源,控制疫情,结合流行病学调查,采集患者、食堂员工相关样本和食堂留样食品进行检测分析,从6份病例样本检出鼠伤寒沙门菌,2份食品样本检出鼠伤寒沙门菌;来自患者和食品的鼠伤寒沙门菌菌株经XbaⅠ酶切后脉冲场凝胶电泳图谱完全一致,快速准确诊断疫情,为及时有效的控制疫情发展提供技术支持。
In June 2013, an outbreak of outbreak of fever, fatigue, headache and diarrhea in Meishan City, Sichuan Province, as the main symptom, occurred. In order to rapidly diagnose the causative agent, find out the source of the pollution, control the epidemic situation, combine the epidemiological investigation, collect the samples of the patients, canteens staffs and the canteens sample food for testing and analysis, detect Salmonella typhimurium from 6 case samples, Salmonella typhimurium was detected in food samples. Salmonella typhimurium isolates from patients and foodstuffs were completely identical by pulsed-field gel electrophoresis after being digested with XbaI. The epidemic situation was quickly and accurately diagnosed, providing technical support for timely and effective control of the outbreak development.