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曾用各种分光光度法测定苄青霉素,但有的专一性差、颜色不稳定,或重视性差。作者用醋酸铜作为发色剂,成功地用于各种药物制剂中苄青霉素的测定。试剂与苄青霉素在pH6～6.8的溶液中按2:1之比形成的绿色络合物于25min 稳定,在650nm 处有最大吸收。苄青霉素浓度在20～1000μg/ml 范围内遵从比尔定律,克分子吸光系数为0.24×10~3lmol/cm。方法简单、快速,20min 内可获得结果。本法与药典方法(碘量滴定法和生物测定法)测得结果一致。方法精密度为1.89%。仪器用Hitachi 139型紫外-可见光分光光度计,1cm 石英比色杯.以蒸馏水溶解标准苄青霉素钾盐(1600U/mg)配成10mg/ml 的标准苄青霉素溶液。用移液管吸取不同体积(0.1～5.0 ml)的苄青霉素溶液(10
Various spectrophotometric methods have been used for the determination of benzyl penicillin, but some are poorly specific, unstable in color, or poorly valued. The authors used copper acetate as a chromogenic agent and successfully used in the determination of benzyl penicillin in various pharmaceutical preparations. Reagents and benzyl penicillin in the pH 6 ~ 6.8 solution at a 2: 1 ratio of the green complex was stable at 25min, maximum absorption at 650nm. Beer’s law was obeyed in the range of 20 ~ 1000μg / ml with the absorbance coefficient of 0.24 × 10 ~ 3lmol / cm. The method is simple and fast, and results are available within 20 minutes. This method and the pharmacopoeia method (iodometric titration and bioassay) measured the same. The precision of the method is 1.89%. Instruments Hitachi 139 UV-visible spectrophotometer, 1cm quartz cuvette. Dissolve standard benzyl penicillin potassium salt (1600U / mg) in distilled water to make a standard 10mg / ml solution of benzyl penicillin. Pipette pipette different volumes (0.1 ~ 5.0 ml) of benzyl penicillin solution (10