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丝蛋白具有良好的生物相容性,生物可降解性以及无免疫原性.利用丝蛋白独特的亲疏水多嵌段共聚物结构特征和构象转变机制,通过乙醇诱导和冷冻相结合的自组装方法制备得到丝蛋白纳米微球后,再在纳米微球表面包覆阿霉素,成功获得了负载阿霉素的丝蛋白纳米载药微球.该载药丝蛋白纳米微球的尺寸为350~400 nm,具有圆球形态并且分散性能良好;其载药率为4.6%,包封率大于90%,在磷酸缓释溶液中的释放可达7天以上.此外,研究发现其缓释行为具有pH响应性,在pH=5.0的磷酸缓冲溶液中的缓释量明显大于在pH=7.4的缓冲液中.体外细胞培养结果显示,纯丝蛋白纳米微球基本没有细胞毒性;而负载有阿霉素的丝蛋白纳米微球能明显抑制癌细胞(Hela细胞)的增殖,且24h和48 h的培养结果表现出与单纯药物相同的药效.因此,该负载阿霉素的丝蛋白纳米微球在临床癌症淋巴化疗方面具有潜在的应用价值.
Silk fibroin has good biocompatibility, biodegradability and non-immunogenicity.Using the structural characteristics and the conformational transition mechanism of silk fibroin, a unique self-hydrophobic multiblock copolymer, a self-assembly method combining ethanol induction and freezing Preparation of silk fibroin nanospheres, and then coated with doxorubicin on the surface of the nanosphere successfully obtained doxorubicin-loaded silk protein nano-loaded microspheres The drug-loaded silk protein nanospheres size of 350 ~ 400 nm, with spherical morphology and good dispersion; its drug loading rate was 4.6%, encapsulation efficiency was greater than 90%, release in phosphate sustained release solution for up to 7 days.In addition, the study found that the sustained release behavior of pH-responsive, the sustained-release amount in phosphate buffer solution at pH = 5.0 was significantly greater than that in buffer solution at pH = 7.4 The in vitro cell culture results showed that pure silk protein nanospheres were almost non-cytotoxic; The results showed that the fibroin nanospheres could obviously inhibit the proliferation of Hela cells, and the results of culturing at 24 h and 48 h showed the same efficacy as that of pure drug alone.Therefore, the loaded doxorubicin-loaded silk protein nanospheres In clinical cancer lymphatic chemotherapy with masks Potential application value.