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目的探讨工厂化培育的北冬虫夏草子实体的动物体内药效学参数。方法健康ICR小鼠或Wistar大鼠分成空白对照组、模型组和给药组,予北冬虫夏草子实体和野生虫草持续给药后,分别检测各组负重持续游泳时间和常压耐缺氧时间、小鼠脾淋巴细胞增殖和NK细胞活性、对CCl4引起的小鼠急性肝损伤的保护作用、对庆大霉素引起的大鼠急性肾损伤的保护作用。结果高剂量北冬虫夏草子实体可延长小鼠持续游泳和常压耐缺氧的平均存活时间至29.05%和31.08%;增加小鼠脾淋巴细胞增殖和NK细胞的活性;明显降低庆大霉素诱发的小鼠肾损伤导致的尿液异常,对BUN和Cr的缓解率分别为71.09%和44.96%,对CCl4诱发的小鼠转氨酶升高,特别是ALT的缓解率为37.82%。结论工厂化培育的北冬虫夏草子实体在保护肝、肾损伤、耐缺氧、抗疲劳以及增强免疫力方面与同剂量野生冬虫夏草相当,值得进一步开发。
OBJECTIVE To investigate the pharmacokinetic parameters of industrialized Cordyceps militaris in vivo. Methods Healthy ICR mice or Wistar rats were divided into blank control group, model group and administration group. After continuous administration to Cordyceps sinensis fruiting body and wild Cordyceps, the duration of sustained weight-bearing swimming and the duration of hypoxia-tolerance were tested, Mouse splenic lymphocyte proliferation and NK cell activity, CCl4-induced acute liver injury in mice, gentamicin-induced acute kidney injury in rats. Results The high dose of Cordyceps sinensis could prolong the average survival time of mice sustained swimming and atmospheric hypoxia to 29.05% and 31.08%, increase the splenic lymphocyte proliferation and NK cell activity in mice, and significantly reduce the effects of gentamycin Of mice with renal damage caused by urine abnormalities, the remission rates of BUN and Cr were 71.09% and 44.96%, respectively. CCl4-induced mice increased transaminases, especially ALT remission rate was 37.82%. Conclusion The industrialized Cordyceps militaris fruiting body is equivalent to wild Cordyceps sinensis in protecting liver and kidney from injury, hypoxia, fatigue resistance and enhancing immunity, so it is worth further development.