A new method for producing a NIAN type wheat maintenance line with the male sterile gene rfv1was described. That is the variety Xinong Fpl, a 1BL/1RS translocation line, as the acceptor and Triticum macha var. subletschchumicum, a non-1BL/1RS transloca-tion line, as the donor, a directional substitution back-cross was made and confirmed by chromosome of root tip preparations and SDS-PAGE analysis. The male ster-ile gene rfv1 of Triticum macha var. subletschchumicum was transferred to the genome of Xinong Fpl. A new NIAN type wheat maintenance line with the male sterile gene rfv1was bred. The method described was success-ful in breeding a new male sterile type for hybrid wheat production.