描述电子传递体参与工作时电流法酶电极的稳态响应特性。根据电子传递体处于溶液中或固定在酶膜中,分别提出数学模型并导出实用的稳态电流关系式。讨论了溶液中传质、酶膜性能和电子传递体本质对响应特性的影响,为传感器酶层设计提供了依据。 Describe the steady-state response characteristics of the current-based enzyme electrode when the electron mediator is involved in the work. According to the electron mediator in solution or immobilized in the enzyme membrane, the mathematical models are respectively proposed and the practical steady-state current relations are derived. The effects of mass transfer, enzyme membrane performance and electron transfer agent in solution on the response characteristics were discussed, which provided the basis for the design of sensor enzyme layer.