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为了研究cpt1基因与水稻根负向光性运动的关系,探讨IAA横向运输与水稻根负向光性运动的关系。检测了外源试剂CaCl2、EDTA以及IAA对水稻根的负向光性运动的效应,并采用RT-PCR的方法验证了不同外源试剂对cpt1基因表达的影响。结果表明,水稻根经光照24h后,与对照相比,1mgL-1的CaCl2和0.001mgL-1的IAA能明显促进水稻根负向光性的弯曲生长,1mgL-1的EDTA(CaCl2的抑制剂)明显抑制水根负向光性的弯曲生长;cpt1基因的表达受上述外源试剂诱导,并表现与水稻根的负向光性生长呈明显的正相关性,推测IAA的横向运输及其运输载体CPT1蛋白对水稻根的负向光性运动具重要作用。
In order to study the relationship between cpt1 gene and rice negative phototaxis and the relationship between IAA lateral transport and rice root negative photomotor activity. The effects of exogenous reagents CaCl2, EDTA and IAA on the negative phototransmission of rice roots were examined. The effects of different exogenous agents on cpt1 gene expression were verified by RT-PCR. The results showed that compared with the control, 1 mg L-1 CaCl2 and 0.001 mg L-1 IAA could significantly promote the growth of rice root negative phototrophy. The inhibitory effect of 1 mg L-1 EDTA (CaCl2 inhibitor ) Significantly inhibited the negative phototrophy growth of watery roots. The expression of cpt1 gene was induced by the above exogenous agents and showed a positive correlation with the negative phototropism of rice roots. It is speculated that the lateral transport and transport of IAA The vector CPT1 protein plays an important role in the negative phototaxis of rice roots.