The effect of glass ionomer cement and resin-modified glass ionomer cement incorporated with chlorhexidine and bioactive glass on antimicrobial activity and physicochemical properties were investigated.The experimental results showed that groups incorporated with 1% chlorhexidine exhibited a significant reduction of optical density values of the bacterial suspension and increased the degradation of Streptococcus mutans biofilm.However,groups incorporated with 10% bioactive glass did not affect the optical density values and the biofilm formation.The mechanical properties of the materials and the polymerization were not influenced by the addition of chlorhexidine.Nevertheless,the compressive strength was lower when the materials were incorporated with bioactive glass.It can be concluded that glass ionomer cements incorporated with chlorhexidine can maintain its mechanical properties as well as reduce early S mutans biofilm formation.Controlled release/sustained release technology may be required to optimize the antibacterial activity of glass ionomer cements incorporated with bioactive glass. The effect of glass ionomer cement and resin-modified glass ionomer cement with chlorhexidine and bioactive glass on antimicrobial activity and physicochemical properties were investigated. The experimental results showed that groups incorporated with 1% chlorhexidine showed a significant reduction of optical density values ​​of the bacterial suspension and increased the degradation of Streptococcus mutans biofilm.However, groups incorporated with 10% bioactive glass did not affect the optical density values ​​and the biofilm formation. The mechanical properties of the materials and the polymerization were not influenced by the addition of chlorhexidine. , the compressive strength was lower when the materials were incorporated with bioactive glass. It could be concluded that glass ionomer cements incorporated with chlorhexidine can maintain its mechanical properties as well as reduce early mutants biofilm formation. Controlled release / sustained release technology may be required to optimize the antibacterial activity of glass ionomer cements incorporated with bioactive glass.