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目的 :探讨白细胞介素 12 (IL 12 )在变应性鼻炎鼻粘膜中的表达。方法 :以卵清蛋白为变应原建立豚鼠变应性鼻炎模型 (模型组 )。取该模型和健康豚鼠鼻粘膜行常规HE染色 (对照组 ) ,并采用逆转录 聚合酶链反应 (RT PCR)检测方法 ,对两组动物鼻粘膜组织中IL 12mRNA表达水平进行相对定量比较。结果 :IL 12mR NA在两组鼻粘膜中均有表达 ,模型组的表达水平为 0 .6 6 7± 0 .10 4显著低于正常对照组 0 .84 7± 0 .0 71(P <0 .0 1)。结论 :在变应性鼻炎鼻粘膜组织中IL 12的表达下降 ,提示应用IL 12替代疗法治疗变应性鼻炎的可能性。
Objective: To investigate the expression of interleukin 12 (IL 12) in nasal mucosa of allergic rhinitis. Methods: The model of allergic rhinitis in guinea pigs was established with ovalbumin as the allergen. The nasal mucosa of this model and healthy guinea pigs were subjected to routine HE staining (control group), and relative quantitative comparison of IL 12 mRNA expression in nasal mucosa tissues was performed by reverse transcription polymerase chain reaction (RT PCR). Results: The expression of IL 12 mRNA in nasal mucosa of both groups was significantly lower than that of the normal control group (0.66 ± 0.104, 0.84 ± 0.71, P <0 .0 1). CONCLUSIONS: The decreased expression of IL 12 in nasal mucosa of allergic rhinitis suggests the possibility of using IL 12 replacement therapy in the treatment of allergic rhinitis.