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本工作主要涉及青蒿琥酯对体外培养人宫颈癌HeLa、Siha细胞株的放射增敏作用。实验选择人宫颈癌细胞HeLa、Siha作为实验细胞。利用四氮唑盐比色试验(MTT)确定细胞辐射损伤实验最佳药物浓度;常规染色体畸变法、胞质分裂阻滞微核法(CB法)分别研究青蒿琥酯对辐射诱发人宫颈癌细胞染色体畸变、微核的影响。确定了青蒿琥酯的实验药物浓度:HeLa为2.0μg/mL、Siha为4.0μg/mL。结果表明,HeLa细胞经青蒿琥酯作用并在相同剂量辐照后,染色体畸变率、微核细胞率及微核率较单纯照射增加明显;Siha细胞在相同辐照剂量下加药处理组与单纯照射组比较,染色体畸变率、微核细胞率及微核率均无统计学差异(p>0.05)。
This work mainly relates to the radiosensitization effect of artesunate on the cultured human cervical cancer HeLa and Siha cell lines in vitro. Human cervical cancer cells HeLa and Siha were chosen as experimental cells. The best drug concentration in cell radiation injury experiment was determined by MTT assay. Conventional chromosome aberration assay and cytosolic blockade micronucleus test (CB method) were used to study the effect of artesunate on radiation-induced human cervical cancer Chromosome aberrations in cells, the effects of micronuclei. The experimental drug concentration of artesunate was determined: HeLa 2.0μg / mL, Siha 4.0μg / mL. The results showed that the chromosome aberration rate, micronucleus rate and micronucleus rate increased significantly in HeLa cells treated with artesunate and irradiated with the same dose of HeLa cells. Siha cells treated with artesunate at the same irradiation dose There was no significant difference in chromosome aberration rate, micronucleus rate and micronucleus rate between the two groups (p> 0.05).